ORAL BIOLOGY AND BIOCHEMISTRY LAB

COMPETENCIES AND TECHNOLOGIES

Detection of biomarkers in biological fluids

  • Enzyme-linked immunosorbent assay
  • Colorimetric and fluorescent-based detection assays

Cell culture

  • Cell viability and cytotoxicity
  • Cell differentiation markers
  • Cell-material interaction studies

Cell morphology analysis

  • Phase-contrast inverted microscopy
  • Fluorescence microscopy

Clinical studies

  • tooth color determination (Spectroshade, Easyshade, eLAB)
  • salivary collection and analysis
  • gingival crevicular fluid collection and analysis

In vitro erosion cycle model

Potentiometry

  • Microelectrode for pH determination in biological fluids
  • Fluoride quantification

Salivary processing and storage

  • Ultracentrifuge
  • Biohood
  • -80ºC freezer
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